Evolution of Tardigrades: Cryptobiotic Survival Strategies

TARDIGRADE CRYPTOBIOTIC
STRATEGIES VS.
MICROGRAVITY
Balaji, Conti, Patel, Souza, Washington
Acknowledgments: Local Partners, Advisors,
and Funders
The Ahmanson Foundation
Dr. James M. Lally
William Saito 
(
Cabinet Office
(Government of Japan),
Ministry of Education - Central Council for
Education)
Dr. Tas Dienes 
(
Chief Technology
Officer at SmartAction)
Martina Ramirez 
(
Loyola Marymount
University)
Ms. Trojanowski
Ms. Curry
Dr. Michael Williams
Professor Jonathan Wright
(Pomona College)
CASIS
Subaru of America, Inc.
Research Team
Co-Principal Investigators
Ashwin Balaji (Grade 12)
Garrett Souza (Grade 12)
David Washington (Grade 12)
Investigator
Richard Conti (Grade 12)
Collaborator
Aaditya Patel (Grade 12)
Teacher Facilitator
Ms. Trojanowski, Damien High School Teacher
Evolution of Tardigrades
 
The ability to undergo
cryptobiosis suggests that
tardigrades evolved in an
unstable and rigorous
environment
Tardigrades cryptobiotic abilities
and tolerance to rigorous
environments suggests they
could have evolved in an
extraterrestrial environment
The Cryptobiotic Process
 
When faced with adverse
environmental conditions
tardigrades shut down all
metabolic processes and enter
into a wrinkled “tun” state, as
seen on the left. Once
reintroduced to hospitable
conditions, the process is
reversed
Initial Ground Truth
Experiment: 20 of 23 specimens
exhibited mobility after 10 days
in a cryptobiotic state
Experiment Procedure
 
Two sets of tardigrades, one
desiccated in moss and one in a
cryptobiotic state on wax paper,
will be sent to the ISS
Another two sets, identical to
those on the ISS, will be studied
on Earth
At the end of the trial period, all
tardigrades  will be returned to
a hydrated state, and their
survival rate will be compared
to that of the ground
experiment
Finding Specimens
Initial procedure used Carolina Biological as a source of tardigrades
After several trials, and contacting the lead biologist at Carolina
Biological, it was determined that the ability to enter into a
cryptobiotic state adapted out of their culture
The specimens used in the experiment were gathered from the
foothills of Glendora, CA
A Baermann funnel was used to isolate the tardigrades from the moss
Three species of tardigrades were native to the Glendora foothills and
used in the experiment: Milnesium tardigradum, Echiniscus testudo,
and Macrobiotus occidentalis
Experimental Implications
 
If tardigrades are able to
survive a prolonged period of
exposure to microgravity, it is
possible that they could have
originated from an
extraterrestrial environment.
Furthermore, if the cryptobiotic
state sufficiently protects
tardigrades from the adverse
conditions of space, this could
present a possible mechanism
by which humans can survive
the longevity and physical rigors
of deep space travel
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Tardigrades, also known as water bears, exhibit fascinating cryptobiotic strategies enabling them to survive extreme conditions. Their ability to undergo cryptobiosis suggests adaptation to unstable environments, possibly even extraterrestrial. Research involving the investigation of tardigrades' cryptobiotic processes and their evolution sheds light on their resilience and adaptability. The experimental procedures involving desiccated and cryptobiotic tardigrades on Earth and the ISS aim to study their survival rates, offering insights into their remarkable survival mechanisms.


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  1. TARDIGRADE CRYPTOBIOTIC STRATEGIES VS. MICROGRAVITY

  2. Acknowledgments: Local Partners, Advisors, and Funders The Ahmanson Foundation Ms. Trojanowski Dr. James M. Lally William Saito (Cabinet Office (Government of Japan), Ministry of Education - Central Council for Education) Ms. Curry Dr. Michael Williams Professor Jonathan Wright (Pomona College) Dr. Tas Dienes (Chief Technology Officer at SmartAction) CASIS Martina Ramirez (Loyola Marymount University) Subaru of America, Inc.

  3. Research Team Co-Principal Investigators Ashwin Balaji (Grade 12) Garrett Souza (Grade 12) David Washington (Grade 12) Investigator Richard Conti (Grade 12) Collaborator Aaditya Patel (Grade 12) Teacher Facilitator Ms. Trojanowski, Damien High School Teacher

  4. Evolution of Tardigrades The ability to undergo cryptobiosis suggests that tardigrades evolved in an unstable and rigorous environment Tardigrades cryptobiotic abilities and tolerance to rigorous environments suggests they could have evolved in an extraterrestrial environment

  5. The Cryptobiotic Process When faced with adverse environmental conditions tardigrades shut down all metabolic processes and enter into a wrinkled tun state, as seen on the left. Once reintroduced to hospitable conditions, the process is reversed Initial Ground Truth Experiment: 20 of 23 specimens exhibited mobility after 10 days in a cryptobiotic state

  6. Experiment Procedure Two sets of tardigrades, one desiccated in moss and one in a cryptobiotic state on wax paper, will be sent to the ISS Another two sets, identical to those on the ISS, will be studied on Earth At the end of the trial period, all tardigrades will be returned to a hydrated state, and their survival rate will be compared to that of the ground experiment

  7. Finding Specimens Initial procedure used Carolina Biological as a source of tardigrades After several trials, and contacting the lead biologist at Carolina Biological, it was determined that the ability to enter into a cryptobiotic state adapted out of their culture The specimens used in the experiment were gathered from the foothills of Glendora, CA A Baermann funnel was used to isolate the tardigrades from the moss Three species of tardigrades were native to the Glendora foothills and used in the experiment: Milnesium tardigradum, Echiniscus testudo, and Macrobiotus occidentalis

  8. Experimental Implications If tardigrades are able to survive a prolonged period of exposure to microgravity, it is possible that they could have originated from an extraterrestrial environment. Furthermore, if the cryptobiotic state sufficiently protects tardigrades from the adverse conditions of space, this could present a possible mechanism by which humans can survive the longevity and physical rigors of deep space travel

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