Enumerate Total WBC Count of Blood: Practical Guide for B.Sc. III Year Students
This practical session on enumerating the total white blood cell (WBC) count of blood provides a detailed procedure using a haemocytometer, sterilized equipment, and Hayem's diluting fluid. Through proper mixing and counting in five different fields, students can calculate the number of WBCs per cubic mm of blood. The step-by-step process and calculation method are outlined, aiding students in understanding and performing the estimation accurately.
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Enumerate The Total WBC Count of Blood Class- B.Sc. III Year Practical Date: 01.10. 2020 Time: 03:00-05:00PM Presented by Manoj Joshi Department of Zoology UCoS,MLSU, Udaipur.
Estimation of WBC Requirements-Haemocytometer, sterilized pricking needle, WBC Pipette, Compound 2.microscope and Hayem s diluting fluid. Procedure- 1. Clean and the mixing pipette. 2. Sterilized your middle finger and Prick the finger and blood flows freely, Don t squeeze. 3. Suck the blood in pipette up to 0.5 ml mark and immediately mix it with Hayem s diluting fluid by sucking it up to 11 mark. 4. Bring counting scale into the focus under objective under microscope. 5. Count cells in 16 squares in five different part of the field.
Calculation- These may be done in the following manner- No. of WBC per cubic mm- Number of cells counted x Dilution x 10 No. of small squares counted Suppose, four medium sized square contain A+B+C+D WBCs. One cubic mm of blood will contain= A+B+C+D x20 x 10 16
