Cleaning and Disinfection Personnel Training Guide

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This training guide focuses on preparing personnel for effective cleaning and disinfection practices. It covers essential steps such as preparation of disinfectant solutions, biosecurity work zones, basic protocol for cleaning, and procedures in the hot zone. It emphasizes the importance of using EPA-registered products, maintaining appropriate contact time, and ensuring all items remain wet with the solution.


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  1. Cleaning and Disinfection Personnel

  2. Personnel Disinfection Station Adjacent to entrance Flat terrain Impermeable surface House components C&D equipment Water supply Waste water containment Cleaning and Disinfection: Personnel Just In Time Training

  3. Preparation Prepare (4) buckets of fresh warm water 1. Add mild detergent/cleaner 2. Rinse water 3. Add EPA-registered disinfectant 4. Rinse water Water temperature no greater than 110oF Running water is preferred Cleaning and Disinfection: Personnel Just In Time Training

  4. Disinfectant Solutions Use according to product label Only EPA-registered or approved products Prepare fresh solutions Old solutions may have reduced efficacy Test kits to help check concentration Cleaning and Disinfection: Personnel Just In Time Training

  5. Biosecurity Work Zones Cleaning and Disinfection: Personnel Just In Time Training

  6. Basic Protocol Systematic manner Cleaning Dry clean Wash Rinse and Dry Disinfection Application Contact time Rinse and Dry Cleaning and Disinfection: Personnel Just In Time Training

  7. Procedure In Hot Zone Dry brush exterior of PPE to remove gross contamination Spray PPE with disinfectant Enter Decon Corridor Cleaning and Disinfection: Personnel Just In Time Training

  8. Wash and Rinse Reusable Clothing Wash items with detergent using soft brush, cloth, or sponge Rinse components with clean, warm water Cleaning and Disinfection: Personnel Just In Time Training

  9. Disinfect Apply EPA-registered disinfectant solution to exterior Allow solution to sit for appropriate contact time Items must remain wet with solution Cleaning and Disinfection: Personnel Just In Time Training

  10. Disposable Clothing Items Spray with EPA-registered disinfectant Allow necessary contact time Doff PPE Place in plastic garbage bags Spray outer surface with disinfectant Place at perimeter for removal Cleaning and Disinfection: Personnel Just In Time Training

  11. Footbaths Ineffective if used incorrectly False sense of security Should not be sole process of disinfection Use fresh solution Allow contact time Cleaning and Disinfection: Personnel Just In Time Training

  12. Respirators C&D procedures available from OSHA Proper cleaning to Reuse respirator Avoid damage to the respirator Avoid harm to user on reuse Cleaning and Disinfection: Personnel Just In Time Training

  13. Respirator C&D Clean Remove filters, cartridges, canisters Disassemble components Wash with warm detergent water Rinse thoroughly Disinfect Immerse in EPA-registered disinfectant Allow needed contact time Rinse thoroughly Change solution after 20 respirators Cleaning and Disinfection: Personnel Just In Time Training

  14. Personnel Shelter for privacy Warm water with antimicrobial soaps Wash hands before leaving Once home (or if possible on-site) Complete shower Cleaning and Disinfection: Personnel Just In Time Training

  15. Safety Chemical Hazards Skin, eye, respiratory irritation Physical Hazards Slips, trips, falls Heat injury High pressure sprayer Cleaning and Disinfection: Personnel Just In Time Training

  16. References https://fadprep.lmi.org USDA APHIS. FAD PReP NAHEMS Highly Pathogenic Avian Influenza Standard Operating Procedures: Cleaning and Disinfection. February 2010. Foot-And-Mouth Disease. Standard Operating Procedures: Cleaning and Disinfection. February 2010. NAHEMS Guidelines: Cleaning and Disinfection. June 2011. Cleaning and Disinfection: Personnel Just In Time Training

  17. Acknowledgments Development of this presentation was by the Center for Food Security and Public Health at Iowa State University through funding from the Multi-State Partnership for Security in Agriculture Author: Glenda Dvorak, DVM, MPH, DACVPM

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