Bacterial Culture Transfer Techniques and Growth Indicators
Bacterial cultures can be transferred using aseptic techniques to ensure purity. Growth indicators such as turbidity in liquid media and colonies on agar plates signify microbial growth. Proper sterilization and handling of instruments are crucial in the process. Techniques like streaking on agar plates and inoculation from stock cultures are commonly used in laboratories for bacterial subculturing.
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Lab7 Culture transfer and techniques The bacteria are (microorganisms), that are growing on or in nutrients media producing what known microbial or bacterial growth or culture when the growth forming from one microorganism is called pure culture, if the growth is formed from more than one microorganism is called mixed culture .Indication of growth in liquid media by turbidity and changing the color of indicator stain. On plate agar by growing colonies. Culture transfer From stock culture (nutrient broth tube or slant tube). From liquid culture (see the figure 18 and 19) All instruments must be sterilized. The work is always under aseptic conditions. The stock a liquous culture must be gently shaked in order to uniform suspending the bacterial cells. Place the stock culture tube and the tube to be inoculated in the palm of one hand (stock culture tube in distal and the inoculated tube in the proximal place).The two tube are the separated to for V-shaped in the hand . By the other hand flame the loop over a Bunsen burner until the wire becomes red-hot. Using the same hand that is holding the inoculating loop, remove the cap, or the cotton plugs from the two tubes and briefly flame their necks over a Bunsen burner by passing them through the flame.(do not allow the tubes to become red hot).
Cool the hot loop in the broth culture .Then transfer 1 drop (loopfull) from stock culture tube into the new broth tube. (By this step could be also transfer to glass slide, streak the surface of a slant agar and streak the bacterial suspension onto surface of plate agar and stab inoculation. Reflame the loop and the necks of the tubes. Recap the tubes. 10-Incubate the inoculated tubes or plates at favorite temperature.
Figure (16) Aseptic technique for bacterial subculturing Figure (17) Transferring technique, a-c stab technique and d - streaking technique
