Animal Use Protocols: Finding the Right Balance

 
Getting it just right
 
Joan T Richerson, MS, DVM, MS, DACLAM
Assistant Chief Veterinary Medical Officer
10/19/21 – AALAS National Meeting – Kansas City, MO
 
 
Getting it just right
 
How is the children’s story of the three bears like an animal
use protocol?
 
Too much and too
specific information
Too little and too vague
information
Just the right amount of
information
 
Porridge
 
Animal Use Protocol
 
 
Getting it just right
 
Example 1:  Intravenous injection
 
Mice will be anesthetized. The tail is secured
;
 a needle and syringe
are used to inject the experimental drug.  Once the mice are
awake, they are returned to their home cages.
 
Getting it just right
 
Example 2:  Intravenous injection
 
Mice will be anesthetized with isoflurane 
(2-5%)
via nose cone inhalation with a
rodent anesthesia machine. Alternatively, 
injectable ketamine or ketamine in
combination with other injectable anesthetics maybe used (see: Appendix 3).
Once the 
mouse
 ceases movement, assessment of the toe pinch reflex and
respiratory rate/depth will ensure adequate anesthesia. The tail will be cleaned
using a 
disinfectant solution (e.g., isopropyl alcohol, betadine, or chlorhexidine)
. The
tail is restrained while occluding one of the two lateral tail veins by applying gentle
pressure. A 26-30 G needle will be inserted into the tail vein and the correct location
confirmed by a flash of blood in the hub when negative pressure is applied to the
syringe. The vein is released, and the 
experimental drug B139
 is injected (see
Appendix 3 for dose range). The needle is withdrawn, and gentle pressure is applied
until the bleeding has stopped. Mice will be observed to ensure adequate
hemostasis, and until the mice are ambulatory.
 
Getting it just right
 
Mice will be anesthetized with 3% isoflurane via nose cone inhalation with a
SomnoSuite™ Low-Flow anesthesia machine. Once the mouse ceases
movement, assessment of the toe pinch reflex and respiratory rate/depth will
ensure adequate anesthesia. The tail will be cleaned using
70% isopropyl alcohol applied with a cotton-tipped applicator. 
The tail will
then be restrained with the ring-finger of the hand holding the mouse; the left
lateral tail vein will be occluded by applying gentle pressure. A 1ml syringe
with a 27G X1/2” needle will be inserted into the left lateral tail vein and the
correct location confirmed by a flash of blood in the hub when negative
pressure is applied to the syringe. The vein is then released, and 10 mg/kg 
of
the 
experimental drug B139
 is injected into the vein. The needle is withdrawn,
and gentle pressure is applied for 5 minutes using a 2”X2” sterile gauze.
Thereafter, mice will be observed every 5 minutes for 20 minutes to ensure
recovery from anesthesia.
 
Example 3:  Intravenous injection
 
Getting it just right
 
Example 3:  Intravenous injection – What’s the problem?
 
Mice will be anesthetized with 
3% isoflurane 
via nose cone inhalation
with a 
SomnoSuite™ Low-Flow
 anesthesia machine. Once the
mouse
 ceases movement, assessment of the toe pinch reflex and
respiratory rate/depth will ensure adequate anesthesia. The tail will
be cleaned using 
70% isopropyl alcohol applied with a cotton-
tipped applicator
. 
The tail will then be restrained with the 
ring-finger
of the hand holding the mice; the 
left lateral tail vein 
will be
occluded by applying gentle pressure. A 
1ml syringe with a 27G
X1/2” needle 
will be inserted into the left lateral tail vein and the
correct location confirmed by a flash of blood in the hub when
negative pressure is applied to the syringe. The vein is then released,
and 
10 mg/kg 
of 
the 
experimental drug B139
 is injected into the
vein. The needle is withdrawn, and gentle pressure is applied for 
5
minutes using a 2”X2” sterile gauze
.  Thereafter, mice will be
observed 
every 5 minutes for 20 minutes 
to ensure recovery from
anesthesia.
 
Getting it just right
 
Being too specific sets an IACUC up for self-made compliance
problems, which could have reporting requirements. Don’t paint
yourself into a corner!
 
Being too vague is also not good but has less risk of self-made
compliance problems but potentially at the expense of humane
care and use.
 
Being descriptive without being too restrictive provides
flexibility with adequate detail to assure humane animal
care and use.
 
Getting it just
right
thank you for
your kind
attention!
 
porridge-illustration-id543212456 (1024×869) (istockphoto.com)
Slide Note
Embed
Share

Exploring the analogy between the story of the three bears and animal use protocols, this content delves into the importance of providing just the right amount of information in research protocols. Through examples of intravenous injections in mice, the text emphasizes the necessity of striking the perfect balance between too much specificity and too little vagueness in experimental procedures. It offers detailed insights into anesthetic methods, drug administration, and post-injection observations to ensure the well-being of research animals while conducting experiments.


Uploaded on Aug 31, 2024 | 0 Views


Download Presentation

Please find below an Image/Link to download the presentation.

The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author. Download presentation by click this link. If you encounter any issues during the download, it is possible that the publisher has removed the file from their server.

E N D

Presentation Transcript


  1. Microsoft clipart, the three bears - Bing images Getting it just right Joan T Richerson, MS, DVM, MS, DACLAM Assistant Chief Veterinary Medical Officer 10/19/21 AALAS National Meeting Kansas City, MO

  2. Getting it just right How is the children s story of the three bears like an animal use protocol? Porridge Animal Use Protocol Too much and too specific information Too little and too vague information Just the right amount of information Microsoft clipart, the three bears and their porridge - Bing images

  3. Getting it just right Example 1: Intravenous injection Mice will be anesthetized. The tail is secured; a needle and syringe are used to inject the experimental drug. Once the mice are awake, they are returned to their home cages.

  4. Getting it just right Example 2: Intravenous injection Mice will be anesthetized with isoflurane (2-5%)via nose cone inhalation with a rodent anesthesia machine. Alternatively, injectable ketamine or ketamine in combination with other injectable anesthetics maybe used (see: Appendix 3). Once the mouse ceases movement, assessment of the toe pinch reflex and respiratory rate/depth will ensure adequate anesthesia. The tail will be cleaned using a disinfectant solution (e.g., isopropyl alcohol, betadine, or chlorhexidine). The tail is restrained while occluding one of the two lateral tail veins by applying gentle pressure. A 26-30 G needle will be inserted into the tail vein and the correct location confirmed by a flash of blood in the hub when negative pressure is applied to the syringe. The vein is released, and the experimental drug B139 is injected (see Appendix 3 for dose range). The needle is withdrawn, and gentle pressure is applied until the bleeding has stopped. Mice will be observed to ensure adequate hemostasis, and until the mice are ambulatory.

  5. Getting it just right Example 3: Intravenous injection Mice will be anesthetized with 3% isoflurane via nose cone inhalation with a SomnoSuite Low-Flow anesthesia machine. Once the mouse ceases movement, assessment of the toe pinch reflex and respiratory rate/depth will ensure adequate anesthesia. The tail will be cleaned using 70% isopropyl alcohol applied with a cotton-tipped applicator. The tail will then be restrained with the ring-finger of the hand holding the mouse; the left lateral tail vein will be occluded by applying gentle pressure. A 1ml syringe with a 27G X1/2 needle will be inserted into the left lateral tail vein and the correct location confirmed by a flash of blood in the hub when negative pressure is applied to the syringe. The vein is then released, and 10 mg/kg of the experimental drug B139 is injected into the vein. The needle is withdrawn, and gentle pressure is applied for 5 minutes using a 2 X2 sterile gauze. Thereafter, mice will be observed every 5 minutes for 20 minutes to ensure recovery from anesthesia.

  6. Getting it just right Example 3: Intravenous injection What s the problem? Mice will be anesthetized with 3% isoflurane via nose cone inhalation with a SomnoSuite Low-Flow anesthesia machine. Once the mouse ceases movement, assessment of the toe pinch reflex and respiratory rate/depth will ensure adequate anesthesia. The tail will be cleaned using 70% isopropyl alcohol applied with a cotton- tipped applicator. The tail will then be restrained with the ring-finger of the hand holding the mice; the left lateral tail vein will be occluded by applying gentle pressure. A 1ml syringe with a 27G X1/2 needle will be inserted into the left lateral tail vein and the correct location confirmed by a flash of blood in the hub when negative pressure is applied to the syringe. The vein is then released, and 10 mg/kg of the experimental drug B139 is injected into the vein. The needle is withdrawn, and gentle pressure is applied for 5 minutes using a 2 X2 sterile gauze. Thereafter, mice will be observed every 5 minutes for 20 minutes to ensure recovery from anesthesia.

  7. Getting it just right Being too specific sets an IACUC up for self-made compliance problems, which could have reporting requirements. Don t paint yourself into a corner! Being too vague is also not good but has less risk of self-made compliance problems but potentially at the expense of humane care and use. Being descriptive without being too restrictive provides flexibility with adequate detail to assure humane animal care and use.

  8. Getting it just right thank you for your kind attention! porridge-illustration-id543212456 (1024 869) (istockphoto.com)

More Related Content

giItT1WQy@!-/#giItT1WQy@!-/#giItT1WQy@!-/#