Polymerase chain reaction - PowerPoint PPT Presentation


Canizzaro Reaction in Organic Chemistry: Experiment and Applications

The Canizzaro reaction involves the disproportionation of aldehydes in the presence of a strong base to produce an alcohol and a carboxylic acid. This experiment, supervised by Lecturer Israa Radhi, explores the mechanism and practical application of the reaction. Benzyl alcohol and benzoic acid, pr

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Aldol Condensation Reaction: Preparation of Chalcones

Chalcones are important unsaturated aromatic ketones that serve as biogenetic precursors of flavonoids and isoflavonoids. They have various medicinal and pharmaceutical applications due to their biological activities. Chalcones are easily synthesized compounds with potential therapeutic uses, making

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Understanding the Diels-Alder Reaction in Practical Organic Chemistry

The Diels-Alder reaction is a fundamental method in organic chemistry for producing cyclic organic compounds by combining a conjugated diene with an alkene. This reaction, named after Otto Diels and Kurt Alder, involves the formation of a six-membered ring with specific bond rearrangements. Conjugat

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Cannizzaro Reaction

The Cannizzaro reaction is a chemical reaction involving the base-induced disproportionation of non-enolizable aldehydes to form a primary alcohol and a carboxylic acid. Discover more about this reaction, its history, mechanism, and variants like the Cross Cannizzaro reaction and Intramolecular Cann

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Benzoin Condensation: A Name Reaction Explained by Dr. Atul Kumar Singh

Benzoin condensation is a classic organic reaction where aromatic aldehydes self-condense to form α-hydroxy ketones. Dr. Atul Kumar Singh, an Assistant Professor of Chemistry, details the mechanism and the specific catalytic properties of cyanide in this reaction. The reaction involves refluxing th

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Investigating Impact of Practice on Human Reaction Time Through Ruler Drop Test

This practical investigation focuses on determining if practice can reduce human reaction times by conducting a ruler drop test. Participants use their weaker hand to catch a ruler dropped by their partner, aiming to improve their reaction time with practice. The experiment explores how athletes can

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Understanding Sanger Sequencing: A Step-by-Step Guide

Explore the detailed procedure of Sanger sequencing, from sample collection to DNA extraction, gene amplification, and sequencing preparation. Learn about the components involved, such as polymerase, nucleotides, and dideoxynucleotides, to achieve accurate DNA sequencing results. Follow the chain-te

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Understanding Polymer Degradation Processes in Chemistry

Polymer degradation involves a reduction in molecular weight due to various factors like heating, mechanical stresses, radiation, oxygen, and moisture. Two main types of degradation include chain end degradation and random degradation, each affecting the polymer structure differently. Chain end degr

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Kinetic Reaction of Sulphite and Iodate - Landolt Reaction Overview

The kinetic reaction of sulphite ions and iodate in the Landolt reaction is a fascinating chemical process where slow and fast reactions occur sequentially, resulting in a visually striking color change. By monitoring the induction period between the two reactions, one can observe the formation of h

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PCR and Blot Techniques in Molecular Biology

Polymerase Chain Reaction (PCR) is a crucial technique for amplifying specific DNA segments rapidly and accurately. Developed in 1983, PCR is widely used in various fields such as molecular biology, forensics, evolution, and medical diagnostics. On the other hand, the Blot technique transfers DNA, R

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Understanding Chemical Kinetics: Rates, Reactions, and Mechanisms

Chemical kinetics involves studying reaction rates, rate laws, stoichiometry, and factors affecting reaction speed. This branch of chemistry delves into determining reaction orders, rate constants, and activation energies using various methods. Different types of rates, such as initial, instantaneou

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Cybersecurity and Supply Chain Risk Management Best Practices

Supply chain attacks pose a significant threat to software developers and suppliers by targeting source codes and build processes to distribute malware. This article discusses the importance of supply chain risk management, the various attack vectors involved, the industries at risk, and the repercu

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Understanding the Prins-Pinacol Reaction in Organic Chemistry

The Prins-Pinacol reaction involves a two-step process starting with the Prins reaction and followed by the Pinacol rearrangement. This reaction, discovered in 1919 by Hendrick J. Prins, is a crucial transformation in organic chemistry, leading to the formation of important carbonyl compounds. The m

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Understanding Kinetics and Reaction Rates in Chemistry

Kinetics is the study of reaction rates and factors affecting them, such as concentration, temperature, catalysts, and more. Orders of reaction classify reactions based on rate dependency on reactant concentration. Factors like pH, light, and solvents can also impact reaction rates. Half-life and sh

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Understanding Free Radical Polymerization Kinetics

This lecture covers the kinetics of free radical polymerization, including initiation, propagation, termination, and kinetic chain length concepts. It explains the calculation of kinetic chain length and chain-transfer reactions. Key points include the rate equations for initiation, propagation, and

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Understanding Chemical Kinetics: Reaction Rates and Mechanisms

Chemical kinetics is a branch of chemistry focused on studying reaction rates and mechanisms. Unlike thermodynamics, which deals with feasibility, kinetics explores the speed at which reactions occur. Factors such as temperature, pressure, and catalysts influence reaction rates. Understanding the ra

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Rutgers Business School Supply Chain Management Curriculum Overview

Explore Rutgers Business School's innovative Supply Chain Management Curriculum designed for high schools. The curriculum focuses on Project-Based Learning (PBL) and integrates essential elements such as significant content, 21st-century skills, in-depth inquiry, and more. The program is based on th

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Introduction to Supply Chain Management

Explore the key components of supply chains, the importance of supply chain management technology, and strategies to overcome challenges. Learn about supply chain visibility, the structure of supply chains, and the three segments - upstream, internal, and downstream. Discover how organizations acces

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Utilizing a Global Model for Analyzing Reaction Pathways in Plasma Systems

This research focuses on using a kinetic global model framework to identify relevant reactions in chemically complex plasma systems. The framework, KGMf, enables the investigation of macroscopic plasma characteristics by analyzing reaction pathways, sensitivity to reaction rate errors, and dominant

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Enhancing Supply Chain Insights Through Holistic Data Synthesis

Synthesizing economic data for comprehensive supply chain analysis, this talk by Krista Chan, Kevin Li, and Christian Moscardi from the U.S. Census Bureau discusses the goals, challenges, supply chain interests, data sources, and desired functionalities to present a holistic view of product supply c

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Understanding Free Energy, Reaction Quotient, and Equilibrium Constant

This educational material delves into the concepts of free energy, reaction quotients, and equilibrium constants in chemical systems. It explains how to determine the direction of a reaction based on Q and K values, elucidates the role of Gibbs free energy in determining spontaneity, and provides ca

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Utilizing Technology for Efficient Health Supply Chain Management in Pakistan During COVID-19

The USAID Global Health Supply Chain Program has supported Pakistan in leveraging its logistics management information system (LMIS) to efficiently plan and deliver critical COVID-19 supplies. Through coordination with government entities and use of various LMIS interfaces, Pakistan has enhanced dat

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Understanding the Value Chain and Supply Chain Dynamics

The value chain involves adding value through a series of activities from producer to consumer, focusing on meeting consumer demands and gaining a competitive advantage. On the other hand, the supply chain focuses on efficient and cost-effective product distribution to meet consumer needs. The prima

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Enhancing Supply Chain Security and IT Governance: An Overview

This presentation delves into the critical aspects of supply chain security and IT governance, highlighting the synchronization of IT decisions across supply chains, global supply chain concerns, the cost implications of supply chain security lapses, and the need for more research and strategic alig

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Understanding Supply Chain Management: Key Concepts and Processes

Supply chain management (SCM) involves the centralized management of goods and services flow, covering processes from raw materials to final products. By efficiently managing the supply chain, companies can reduce costs and improve product delivery. This seminar presentation explores the definition,

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Cloning Other Genes and Recombinant DNA Technology

The recombinant vector with a kanamycin resistance gene can be used to clone other genes by inserting human DNA fragments and selecting transformed E. coli cells. This technique has enabled the production of various human proteins for therapeutic purposes, such as insulin, growth hormones, and clot-

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Understanding DNA Transcription and Translation in Biology

DNA transcription is the process where DNA is used as a template to create mRNA in the nucleus. This mRNA is complementary to the DNA and goes through initiation, elongation, and termination stages. RNA polymerases are essential for this process in eukaryotes. Various RNA polymerases have specific f

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Overview of RNA Transcription Process

The process of RNA transcription involves three main stages: initiation, elongation, and termination. Initiation starts with RNA polymerase binding to a promoter, followed by the formation of a transcription initiation complex. Elongation involves RNA polymerase untwisting the DNA helix and adding n

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Understanding RNA Polymerases and Transcription Process

RNA polymerases play a crucial role in synthesizing cellular RNA through transcription, where RNA is created from a DNA template. This process involves specific requirements such as a DNA template, ribonucleoside triphosphates, and divalent metal ions. RNA polymerase catalyzes the initiation and elo

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Enhancing Supply Chain Efficiency with Quality Systems

Explore the best practices and methodologies for improving supply chain operations such as planning, sourcing, making, delivering, and returning. Discover key initiatives like E-procurement, bar coding, supplier quality assurance, and green supply chain to elevate functional excellence. Align supply

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Grignard Reaction in Chemistry Lab: Part 1 Overview

The Grignard Reaction Part 1 in Chemistry 318 Fall 2018 involves the preparation of the Grignard reagent, its reaction with CO2, and the isolation of the benzoic acid product. The experiment spans two lab sessions, focusing on safety precautions, pre-lab checks, and upcoming due dates. Students are

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Introduction to Chemical Reaction Engineering (CRE)

Chemical Reaction Engineering (CRE) focuses on studying the rates and mechanisms of chemical reactions, as well as designing reactors for these reactions. The field involves understanding balances in terms of molar flow rates, mole balances, rate laws, stoichiometry, and membrane reactors. Membrane

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China-Africa Supply Chain Cooperation: Challenges and Opportunities

China-Africa Supply Chain Cooperation presents both challenges and opportunities for development. The growth of China-Africa supply chain is crucial, considering Africa's participation in the global supply chain mainly focused on providing primary products. The strategic importance of this relations

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Overview of Thermostable DNA Polymerases and Their Properties

Thermostable DNA polymerases play a crucial role in various molecular biology techniques, with Taq DNA polymerase being a household name due to its discovery in Thermus aquaticus. These enzymes catalyze DNA synthesis, require magnesium ions for activity, and exhibit optimal function at high temperat

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Understanding Polymerase Chain Reaction (PCR) in Genetic Engineering

Polymerase Chain Reaction (PCR) is a key enzymatic method in genetic engineering developed in 1983. It amplifies targeted regions of DNA, aiding in various applications like studying diseases, forensic analysis, and analyzing ancient DNA. PCR involves heating, denaturation, primer binding, and exten

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Understanding the Redox-Relay Heck Reaction in Organic Synthesis

The Redox-Relay Heck Reaction is a powerful tool in organic synthesis that allows for the functionalization of olefins with aryl groups. Developed by Sigman and colleagues, this reaction involves a palladium-catalyzed relay controlled by a thermodynamic sink, leading to the formation of aldehydes or

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Understanding Chemical Kinetics: Reaction Rates and Activation Energy

Exploring the fundamental concepts of chemical kinetics, this content delves into reaction rates, collision theory, and activation energy in chemical reactions. It emphasizes the importance of particle collisions, correct orientation, and energy requirements for reactions to occur. Through energy di

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Understanding DNA Replication Process in Living Organisms

DNA replication is a fundamental biological process where an original DNA molecule produces two identical copies. This process involves initiation, elongation, and termination stages, utilizing replicator and initiator proteins. The DNA is unwound and replicated with the help of enzymes like helicas

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Enhancing Nepal's Agricultural Supply Chain for Sustainable Growth

Nepal's agricultural imports, dominated by staples, edible oil, vegetables, fruits, and more, indicate the need to strengthen the agricultural supply chain. Analyzing trade indicators reveals a trade deficit and the importance of optimizing supply chain management. The country's fragmented ASC manag

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Exploring Exponential Growth in Polymerase Chain Reaction (PCR) for Novel Product Production

Delve into the fascinating world of Polymerase Chain Reaction (PCR) to understand exponential growth in product production using molecular biology and mathematical concepts. High school biology standards, algebraic models, and learning goals are intertwined to predict product amounts and assess modi

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