Glycoproteins play a significant role in biopharmaceuticals, necessitating detailed characterization due to their structural heterogeneity from glycosylation. This study focuses on developing a robust and cost-effective characterization method using a Definitive Screening Design to optimize an HPAE-

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Time curves illustrating sialylation, bisection, fucosylation, and DAS28-CRP disease activity in pregnant rheumatoid arthritis patients based on ACPA IgG galactosylation. The figures show changes in glycosylation patterns across IgG subclasses and disease activity in ACPA-positive and ACPA-negative

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