Blood Spatter Analysis Training Materials - Updated 2023

 
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CC BYThis Photo
CSI Unit Training Materials
 
T. Tomm Updated 2023
https://sciencespot.net
 
T
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The student digital slides for this unit are included with the
file available at
2TPXZvFgH3nvMom8tL-sgAa8PLXM0/edit?usp=sharinghttps://docs.google.com/presentation/d/1cK42rhAWr0iTKZ
 
IMPORTANT:  Make a copy of the student notebook
before you assign it on your LMS.  It is view-only and I am
not able to approve access for your students.
 
The digital notebook (with all the slides for the digital
lessons and activities) is available at
.
It is set up for standard paper (8.5x11) in landscape mode
for easier printing when needed.  Paper versions are
available for this unit on my Forensic Science page at
https://sciencespot.net/Pages/classforsci.htmlyuUxiEsSM2m4YOAtIlfmD4O4YsLdPI/edit?usp=sharinghttps://docs.google.com/presentation/d/14eIEiGacRXMsD
A DRAFT of the general outline of the digital lessons (with
corresponding slide #s for the student notebook) is
available at 
 .  It is based on 44-minute class
periods with 7
th
 & 8
th
 grade students.
9mA/edit?usp=sharinguHQPXDKOxmZXtU_gxy9dphEuyC1P4jzP-https://docs.google.com/document/d/15wxjmL-
 
Preview presentations before using with your classes as
many slides have animations and links you will need to
use.
 
I do Science Starters at the beginning of each class
period.  A collection of starters for Forensics is available
on my lessons plans page at
 .
https://sciencespot.net/Pages/classforsci.html
 
Part A:  Watch the 
Blood Spatter 101 
video to help you
complete this section.  
1.  What does BPA represent?
2. What are the three types of blood patterns?
_____________ (or drip) stains occurs when gravity
pulls blood downward to the floor or surface. They
tend to be round and fat without tails.
______________ stains occurs when a bloody object
comes in contact with another object or surface;
appears as smears or stripes
_______________ (or ___________) patterns occur
when a force is applied to a blood object or pool and
are classified as high, medium, or low velocity.
B
loodstain 
P
attern 
A
nalysis
PASSIVE
TRANSFER
IMPACT
Note:  Due to age restrictions, video 
#1 is
only available on YouTube.
B
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3. What can the shape and size of a droplet tell us?
The angle of impact and the
speed the droplet was traveling.
Examine the blood spatter samples on your table.  What do you
observe?
I made several sample pages of
blood spatter based on the four
labs in this lesson.  I laminated
the pages & places several on
each table.
 
Part B: Watch the 
BLOOD SPATTER BASICS 
video to
help you complete this section.
1. The trainers created _________ scenes using ___________ blood to
help police officers learn how to investigate crime scenes with blood
evidence.
2.  The height of the drop as well as the speed at which it is traveling
affect the ________ and _________ of the blood droplets.
3. True or False?
____  Blood evidence follows a definite pattern that can be applied to
          every crime  scene.
____ Popular television shows, such as CSI Miami, are an accurate
         representation of how investigators analyze real crime scenes.
 
____  Blood evidence is easy to analyze and always tells investigators
          who is responsible for the crime.
4. The trainer reminds the investigators that what they can't ________,
they can't _____ in a case or court of law.
MOCK
ANIMAL
SIZE
SHAPE
F
F
F
PROVE
SAY
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Part C: What else can an investigator learn from the
analysis of a blood spatter?
TYPE OF INJURY
- Blunt force, sharp force, gunshot, etc.
WEAPON
 - Type and velocity
 WOUNDS
 – Number of blows or the order in which they
were inflicted
POSITIONS
 and 
MOVEMENTS
 of the victim and
suspect during the attack
Source: http://science.howstuffworks.com/bloodstain-pattern-analysis1.htm
https://www.youtube.com/watch?v=I0-nIsw1LXU
How does a blood
droplet form?
Click the image
for more details.
Light Source
Investigators will first examine the crime scene to look for areas
that may contain blood.  They may use high-intensity light or UV
lights to help them find traces of blood as well as other  bodily
fluids that are not visible under normal lighting conditions.
Part D: How is blood evidence detected at a crime scene?
Blood Reagent Tests
These tests, referred to as presumptive tests, are used to detect
blood at crime scenes based upon the properties of hemoglobin in
the blood.  Further tests at the crime lab can determine if it is
human  blood or not.
Examples
:
 Phenolphthalein
 is a chemical that is still utilized today and is
usually referred to as the Kastle-Meyer test and produces a pink
color when it reacts with hemoglobin.
HemaStix
 is a strip that has been coated with
tetramethylbenzidine (TMB) and will produce a green or blue-
green color with the presence of hemoglobin.
Kastle-Meyer Test
Video
HemaStix
Luminol
This chemical is used by crime scene investigators to locate traces of
blood, even if it has been cleaned or removed.
Investigators spray a luminol solution is throughout the area under
investigation and look for reactions with the iron present in blood, which
causes a blue luminescence.
One problem is that other substances also react, such as some metals,
paints, cleaning products, and plant materials. Another problem is that the
chemical reaction can destroy other evidence in the crime scene.
LCV
 or 
Leuco Crystal Violet
, is one type of chemical process that is used for blood
enhancement.  Using this test helps to make the blood evidence more 
visible
 so it can be
photographed and analyzed.
Fluorescein
This chemical is also capable of detecting latent or old blood, similar to
luminol. It is ideal for fine stains or smears found throughout a crime
scene. After the solution has been sprayed onto the substance or area
suspected to contain blood, a UV light and goggles are used to detect any
illuminated areas, which appear greenish-white if blood is present. It may
also react to many of the same things as luminol (copper and bleach).
Fluorescein
Reaction  in
UV Light
Part E: Bloodstain Pattern Analysis Terms
Bloodstains created from the application of force to
the area where the blood originated.
The place from where the blood spatter came from
or originated.
Small drops of blood that break off
from the parent spatter
The pointed edges of a stain that
radiate out from the spatter
The droplet from which a satellite
spatter originates.
SPATTER
SATELLITE
SPINES
PARENT DROP
ORIGIN/
SOURCE
Part F: Blood Spatter Labs
 
 
 This can be messy!  Be very careful to keep the blood on
the paper and not on yourself, the table, or floor.
 Hold you hand as steady as possible when making the
drops.  Brace your wrist against the meter stick to help
you.
 Get a pair of goggles and an apron.  Sit quietly until we
are ready to continue!
If you make a mess or goof up a drop,
grab a paper towel and wipe it up immediately!
1.
Divide the paper into 
THREE
sections.
2.
Label 
one side 
with “
30 S
” for
30 cm 
and 
SINGLE
 droplet.
3.
Label the 
other side 
with “
60 S
for 
60 cm 
and 
SINGLE
 droplet.
4.
Label the 
middle
 
with “
90 S
for 
90 cm 
and 
SINGLE
 droplet.
Getting ready …
Lab #1:  SINGLE DROPS
 
FOR EACH HEIGHT:
-
 Hold the dropper bottle upside down (& away
from the ruler) so that the 
BOTTOM
 of it is the
correct height from the table and blood won’t get
on the meterstick.
-
 GENTLY
 squeeze the bottle so that 
ONE
 drop
of blood is released and lands on your paper.
 Complete 
THREE
 good drops at each height.
If you make a mess or goof up a drop,
grab a paper towel and wipe it up immediately!
The higher the height of the drop, the _________ the diameter
of the parent drop and the ___________ the spines become.
WIDER
LONGER
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What did you notice about the droplets (size, shape, & spines) as you increased the  height
of the drop? Explain using the terms height, diameter, and spines in your answer.
Lab #2:  MULTIPLE DROPS
 
Directions
:
 You are going to repeat the process from Lab
1 - same labels, same process.
You need to have 
TWO
 droplets in the
SAME SPOT
.   If you miss, wipe it off with a
towel and try again.
 Try 
THREE TIMES 
from 
THREE
HEIGHTS 
– 30 cm, 60 cm, and 90 cm – with
the simulated blood.
If you make a mess or mess up a drop,
grab a paper towel and clean it up immediately!
 
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What did you notice about the 
droplets (size, shape, & spines) as you increased the height
of the drop? Explain  using the terms height, diameter, spines, and satellites in your answer.
How do your results from Lab 2 compare to Lab 1?
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MORE
OVAL
SPINES
The single drops from Lab 1 did not have _____________ spatter
and the spines tended to be ___________.
SATELLITE
SHORTER
WIDER
 
 We are going to walk at different rates (slow, medium,
and fast) along a strip of paper and observe the changes in
the spatter.
Some of you will have a marker so you can help label the
spatter.
 Use S for slow, M for medium, and F for fast.
Lab #3:  MOTION DROPS
 
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The __________ a person is moving the more-rounded the
droplets with spines on all sides. The droplets will also be
________ _____________.
As a person’s speed _____________, the droplets will be
further apart with an _______ shape and spines mostly on
_____ side.
SLOWER
INCREASES
OVAL
ONE
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What did you notice about the droplets (size, shape, & spines) as you increased the walking
speed?  Explain using the terms speed, shape (round/oval), distance, and spines.
CLOSER TOGETHER
 
 
Directions:
 
 Divide a piece of copy paper into three columns and
label with the angle for each droplet - 
25
o
, 45
o
, 60
o
.
 Place the paper on the clip board and 
align the
clipboard with the 25
o
 line
. Hold the bottle of blood
at a height of 
50 centimeters 
from the 
top of the
paper
.
 GENTLY squeeze the bottle so that a 
SINGLE 
drop
of blood is released and lands on the paper.  
Repeat
two
 more times 
at this angle.
 Try the test again, but use 45
o
 and 60
o
Lab #4:  ANGLE OF IMPACT
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How does the ANGLE OF IMPACT affect the blood spatter?  Explain using the terms angle,
shape, length, & tails.
 
The greater the angle with the table (25
o
 
 60
o
), 
the ___________
the droplet and more ________-shaped it will be with _________
tails.
The droplets that form from angles close to 90
o
 with the table tend
to be __________-shaped without __________.
LONGER
OVAL
LONGER
ROUND
TAILS
Has spines on all sides – length
depends on the height of the drop
Indicates the direction of movement
after the droplet hits the surface
 
Remember the blood spatter samples on your table at the start of
this lesson.  Examine them again.
Are you able to explain how each of the patterns was likely
created?
 
 
 
 
How did your observations change after completing the labs?
 
I put the same sample pages
from the start of the lesson on
the table for students to observe
again.  They are usually able to
explain the most likely
cause/process that formed each
one.
 
3. If you find a trail of blood with droplets that are round and close
together with small spines, what could this mean?
 
 
4. How do Newton’s Laws of Motion apply to blood spatter?
 
These questions
are listed to the
right of the student
slide from the
spatter labs.
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Learn about bloodstain pattern analysis, types of blood patterns, droplet analysis, and investigative techniques through comprehensive training materials. Explore the significance of blood spatter in crime scene investigations and understand how investigators derive crucial information from blood evidence.


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  1. CSI Unit Training Materials T. Tomm Updated 2023 https://sciencespot.net

  2. Part A: Watch the Blood Spatter 101 video to help you complete this section. Bloodstain Pattern Analysis 1. What does BPA represent? 2. What are the three types of blood patterns? PASSIVE _____________ (or drip) stains occurs when gravity pulls blood downward to the floor or surface. They tend to be round and fat without tails. TRANSFER ______________ stains occurs when a bloody object comes in contact with another object or surface; appears as smears or stripes SPATTER IMPACT _______________ (or ___________) patterns occur when a force is applied to a blood object or pool and are classified as high, medium, or low velocity. Click to reveal answers

  3. 3. What can the shape and size of a droplet tell us? The angle of impact and the speed the droplet was traveling. Examine the blood spatter samples on your table. What do you observe?

  4. Part B: Watch the BLOOD SPATTER BASICS video to help you complete this section. MOCK ANIMAL 1. The trainers created _________ scenes using ___________ blood to help police officers learn how to investigate crime scenes with blood evidence. 2. The height of the drop as well as the speed at which it is traveling affect the ________ and _________ of the blood droplets. SIZE SHAPE 3. True or False? ____ Blood evidence follows a definite pattern that can be applied to every crime scene. ____ Popular television shows, such as CSI Miami, are an accurate representation of how investigators analyze real crime scenes. ____ Blood evidence is easy to analyze and always tells investigators who is responsible for the crime. 4. The trainer reminds the investigators that what they can't ________, they can't _____ in a case or court of law. SAY F F F PROVE

  5. Part C: What else can an investigator learn from the analysis of a blood spatter? TYPE OF INJURY- Blunt force, sharp force, gunshot, etc. WEAPON - Type and velocity WOUNDS Number of blows or the order in which they were inflicted POSITIONS and MOVEMENTS of the victim and suspect during the attack https://www.youtube.com/watch?v=I0-nIsw1LXU Source: http://science.howstuffworks.com/bloodstain-pattern-analysis1.htm

  6. Part D: How is blood evidence detected at a crime scene? Light Source Investigators will first examine the crime scene to look for areas that may contain blood. They may use high-intensity light or UV lights to help them find traces of blood as well as other bodily fluids that are not visible under normal lighting conditions. Blood Reagent Tests These tests, referred to as presumptive tests, are used to detect blood at crime scenes based upon the properties of hemoglobin in the blood. Further tests at the crime lab can determine if it is human blood or not. Kastle-Meyer Test Video Examples: Phenolphthalein is a chemical that is still utilized today and is usually referred to as the Kastle-Meyer test and produces a pink color when it reacts with hemoglobin. HemaStix is a strip that tetramethylbenzidine (TMB) and will produce a green or blue- green color with the presence of hemoglobin. has been coated with HemaStix

  7. Luminol This chemical is used by crime scene investigators to locate traces of blood, even if it has been cleaned or removed. Investigators spray a luminol solution is throughout the area under investigation and look for reactions with the iron present in blood, which causes a blue luminescence. One problem is that other substances also react, such as some metals, paints, cleaning products, and plant materials. Another problem is that the chemical reaction can destroy other evidence in the crime scene. Luminol Reaction Fluorescein This chemical is also capable of detecting latent or old blood, similar to luminol. It is ideal for fine stains or smears found throughout a crime scene. After the solution has been sprayed onto the substance or area suspected to contain blood, a UV light and goggles are used to detect any illuminated areas, which appear greenish-white if blood is present. It may also react to many of the same things as luminol (copper and bleach). Fluorescein Reaction in UV Light LCV or Leuco Crystal Violet, is one type of chemical process that is used for blood enhancement. Using this test helps to make the blood evidence more visible so it can be photographed and analyzed.

  8. Part E: Bloodstain Pattern Analysis Terms Bloodstains created from the application of force to the area where the blood originated. SPATTER ORIGIN/ SOURCE The place from where the blood spatter came from or originated. Small drops of blood that break off from the parent spatter SATELLITE SPINES The pointed edges of a stain that radiate out from the spatter The droplet from which a satellite spatter originates. PARENT DROP

  9. Part F: Blood Spatter Labs This can be messy! Be very careful to keep the blood on the paper and not on yourself, the table, or floor. Hold you hand as steady as possible when making the drops. Brace your wrist against the meter stick to help you. Get a pair of goggles and an apron. Sit quietly until we are ready to continue! If you make a mess or goof up a drop, grab a paper towel and wipe it up immediately!

  10. Getting ready 1. Divide the paper into THREE sections. 30 S 2. Label one side with 30 S for 30 cm and SINGLE droplet. 90 S 3. Label the other side with 60 S for 60 cm and SINGLE droplet. 60 S 4. Label the middle with 90 S for 90 cm and SINGLE droplet.

  11. Lab #1: SINGLE DROPS FOR EACH HEIGHT: X - Hold the dropper bottle upside down (& away from the ruler) so that the BOTTOM of it is the correct height from the table and blood won t get on the meterstick. - GENTLY squeeze the bottle so that ONE drop of blood is released and lands on your paper. Hold the bottle away from the meterstick Complete THREE good drops at each height. If you make a mess or goof up a drop, grab a paper towel and wipe it up immediately!

  12. Lab 1: SINGLE DROPS The answers are What did you notice about the droplets (size, shape, & spines) as you increased the height of the drop? Explain using the terms height, diameter, and spines in your answer. WIDER The higher the height of the drop, the _________ the diameter of the parent drop and the ___________ the spines become. LONGER Quick Question: Which of the three blood droplets shown would have been created by a wound in the lower part of the leg? Why? The smallest diameter would be the from a lower height.

  13. Lab #2: MULTIPLE DROPS Directions: 30M 90M 60M You are going to repeat the process from Lab 1 - same labels, same process. X You need to have TWO droplets in the SAME SPOT. If you miss, wipe it off with a towel and try again. Try THREE TIMES from THREE HEIGHTS 30 cm, 60 cm, and 90 cm with the simulated blood. Hold the bottle away from the meterstick, but brace your hand so it doesn t move. If you make a mess or mess up a drop, grab a paper towel and clean it up immediately!

  14. DONE with Lab #2? Fold up your blood spatter paper and throw away. Put metersticks and blood on front counter Wash your hands. Answer the questions for Lab #2 before class tomorrow.

  15. Lab 2: MULTIPLE DROPS The answers are What did you notice about the droplets (size, shape, & spines) as you increased the height of the drop? Explain using the terms height, diameter, spines, and satellites in your answer. WIDER The more droplets that land in the same place, the ___________ the diameter of the spatter pattern and _________ satellites appear that are _______-shaped with some with _________. OVAL MORE SPINES How do your results from Lab 2 compare to Lab 1? SATELLITE The single drops from Lab 1 did not have _____________ spatter and the spines tended to be ___________. SHORTER

  16. Lab #3: MOTION DROPS We are going to walk at different rates (slow, medium, and fast) along a strip of paper and observe the changes in the spatter. Some of you will have a marker so you can help label the spatter. Use S for slow, M for medium, and F for fast. Answer the questions for Lab #3 before class tomorrow.

  17. Lab 3: MOTION DROPS The answers are What did you notice about the droplets (size, shape, & spines) as you increased the walking speed? Explain using the terms speed, shape (round/oval), distance, and spines. SLOWER The __________ a person is moving the more-rounded the droplets with spines on all sides. The droplets will also be ________ _____________. CLOSER TOGETHER INCREASES OVAL As a person s speed _____________, the droplets will be further apart with an _______ shape and spines mostly on _____ side. ONE

  18. Lab #4: ANGLE OF IMPACT Directions: Divide a piece of copy paper into three columns and label with the angle for each droplet - 25o, 45o, 60o. Place the paper on the clip board and align the clipboard with the 25o line. Hold the bottle of blood at a height of 50 centimeters from the top of the paper. GENTLY squeeze the bottle so that a SINGLE drop of blood is released and lands on the paper. Repeat two more times at this angle. Try the test again, but use 45o and 60o

  19. Lab 4: ANGLE OF IMPACTThe answers are How does the ANGLE OF IMPACT affect the blood spatter? Explain using the terms angle, shape, length, & tails. The greater the angle with the table (25o the droplet and more ________-shaped it will be with _________ tails. 60o), the ___________ LONGER LONGER OVAL The droplets that form from angles close to 90o with the table tend to be __________-shaped without __________. ROUND Has spines on all sides length depends on the height of the drop TAILS What does the tail tell us? Indicates the direction of movement after the droplet hits the surface

  20. Remember the blood spatter samples on your table at the start of this lesson. Examine them again. Are you able to explain how each of the patterns was likely created? How did your observations change after completing the labs?

  21. 1. What forces are acting on a blood droplet as it falls? 2. If you have a blood droplet like this one, what does it tell you? Explain. QUICK QUESTIONS 3. If you find a trail of blood with droplets that are round and close together with small spines, what could this mean? 4. How do Newton s Laws of Motion apply to blood spatter?

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