Understanding Cauliflower Mosaic Virus: Structure, Reproduction, and Genome
Cauliflower Mosaic Virus (CaMV) is a plant virus studied by Assistant Professor Kavita Basumatary at Goalpara College. The virus has a circular, double-stranded DNA genome of about 8000 bp in length with unique discontinuities. Reproduction involves the viral genome entering the host cell nucleus, where minichromosomes are formed to produce essential RNAs. Replication of the viral genome is a crucial step in the viral life cycle. This comprehensive study delves into the intricate structure and mechanisms of CaMV.
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CAULIFLOWER MOSAIC VIRUS KAVITA BASUMATARY ASSISTANT PROFESSOR DEPARTMENT OF BOTANY GOALPARA COLLEGE
Structure of Viral Genome: The CaMV genome consists of circular, double- stranded DNA approximately 8000 bp in length. The genome contains three discontinuities typical of pararetroviruses. There are seven open reading frames (ORFs) which code for six proteins of known function; P1-P6. Two main RNAs are produced from cccDNA: pregenomic 35S RNA and subgenomic 19S RNA.
Reproduction of Virus: The virion enters the host cell, moves to the nuclear pore, and the viral genome is imported in the nucleus. Discontinuities in the genomes are repaired to form minichromosomes. Minichromosomes are used as templates to produce 35S RNA & 19S RNA. P6 is translated from 19S RNA and accumulates in electron-dense inclusion bodies (edIBs) P6 transactivates production of P1-P5 from 35S RNA Packaging occurs. The virion may either reinfect the nucleus to increase the copy minichromosome (early in the infection) or it may leave the cell. number of the
