The Importance of Flavor in Meat Science

 
Meat Flavor
 
Jerrad Legako
Graduate Student, Meat Science
 
 
ANSC 3404
 
IMPORTANCE OF FLAVOR
 
Consumer perception of meat quality
After appearance and tenderness flavor is most
important
Specifically cooked meat flavor
Flavor is a key factor for acceptance
Understanding flavor is therefore crucial
Analysis of flavor compounds
 
 
 
 
   
(Machiels and Istasse)
 
Tenderness
Juiciness
Flavor
Flavor often considered the most important when tenderness is
acceptable
Result of volatile compounds produced during cooking
 
MEAT PALATABILITY
 
TASTE vs. FLAVOR
 
 Taste refers to the five basic receptors: sweet,
salty, sour, bitter and umami
 Flavor is the perception of chemical
compounds reacting with receptors in the oral
and nasal cavities (aroma) in combination with
taste
Olfactory 
receptors
Taste receptors
Posterior 
nares
Anterior 
nares
Tongue
Perception of Flavor (Taste and Aroma)
(L. J. Farmer)
 
TASTE
 
 
 Salt and sour receptors are well
understood while bitter and
sweet receptors appear to be
more complex.
 
UMAMI
 
Umami [oo-MOM-ee], known as the fifth taste,
is described as meaty and savory or delicious
derived from 
umai
, the Japanese word for delicious
Glutamates – the salts of an amino acid - and other small
molecules called nucleotides
Although umami has been known for quite
awhile, recently umami receptors have been
clearly identified so this is a bona fide fifth taste.
 
5 TASTES
 
 Detection of these five tastes has been key to
our survival throughout the ages
Sweet means energy-giving carbohydrates
 Salt indicates essential minerals for life-sustaining cell
functions and wound healing
 Sour says to “proceed with caution,” since many foods sour
as they deteriorate.
Umami signifies life-giving protein.
And bitter warns “spit it out, don’t touch it” because many
natural toxins taste bitter
 
FLAVOR CHEMISTRY
 
What is flavor chemistry?
A study of compounds which elicit a
chemosensation or flavor sensation
 
What produces flavor sensations?
Chemical compounds reacting with
receptors in the oral and nasal cavities
Specifically ingested 
volatile
compounds
 
 
           
(Christen and Smith)
 
FLAVOR CHEMISTRY
 
Over 200 flavor compounds associated with
cooked beef
Sulferous and carbonyl compounds are predominate
contributors
Maillard reaction products
End products result from sugars and amino groups
Lipid breakdown products
Higher concentrations may produce undesirable flavors
 
(Calkins and Hodgen)
MAIN FLAVOR FORMING REACTIONS
 
P
P
r
r
e
e
c
c
u
u
r
r
s
s
o
o
r
r
s
s
A
A
r
r
o
o
m
m
a
a
 
 
c
c
o
o
m
m
p
p
o
o
u
u
n
n
d
d
s
s
,
,
 
 
e
e
.
.
g
g
.
.
 
MAILLARD REACTION
 
 Reducing sugar and free amino acid
Results in the production of H
2
O,
Therefore reaction does not occur with moist heat cookery
 
reducing sugar + amine = brown pigments + flavors
 
Flavor Compounds Formation by Maillard
Reaction
 
Reducing Sugars and 
-amino acids
 
N-glycosylamine or N-fructosylamine
 
1-Amino-1-deoxy-2-ketose (Amadori intermediate) or
2-Amino-2-deoxy-1-aldose (Heynes intermediate)
 
Reductones and Dehydroreductones
 
Furans
Thiophenes
Pyrroles
 
Retroaldol Reaction
 
H
2
S
NH
3
 
Strecker
degradation
 
Amino
Acids
 
Hydroxyacetone
Hydroxyacetylaldehyde
Acetoin
Acetylaldehyde
 
Glyoxal
 Pyruvaldehyde
 Glycerolaldehyde
 
Strecker Aldehydes 
+
CO
2
 
+ 
-aminoketone
(Methional, NH
3
, H
2
S)
 
Heterocyclizaion
 
Pyrazines
Pyridines
Oxazoles
 
Thiazoles
Pyrroles
 
+
 
+
 
FLAVOR COMPOUND ANALYSIS
 
Volatile compounds must be extracted
Head space solid phase microextraction (HS-SPME)
Separation of compounds may be done via gas
chromatography (GC)
Compounds may then be identified and sensory evaluated
Identified: purified standards and/or mass spectral library
Sensory evaluation: olfactometry
 
(Frank and others)
 
HS-SPME/GC-MS-Olfactory
 
DISCUSSION
 
 
Flavor is important to overall palatability of meat
Flavor compounds may be studied by chemical and sensory
evaluation
Maillard reaction and lipid breakdown products are dependent
on the type and condition of meat
 
 
Species
Red meat species and poultry
Breed
Bos indicus vs Bos taurus?
Sex
Androstenone, skatole
Diet
Grain-fed vs. Grass-fed
Age
Young lamb vs. mutton
Packaging
MAP, over-wrap, vacuum
 
FACTORS AFFECTING MEAT FLAVOR
 
Fat
Amount and type
Muscle
Location effect
Aging
Dry vs. wet
Enhancement
Brine solution containing salt
Cooking method
Dry vs. moist heat
Degree of doneness
 
FACTORS AFFECTING MEAT
FLAVOR
 
Top 20 Beef Flavor Pairings in
Newsstand Beef Recipes
 
•  Onion 60%
 •  Garlic 55%
•  Tomato* 32%
•  Beef Broth/Stock/Bouillon* 25%
•  Wine (mainly red)* 20%
•  Cheese (mainly Parmesan, Cheddar, Blue)* 19%
•  Sugar 18% 
 
•  Cream 18%
•  Bell Pepper 16% 
 
•  Vinegar 16%
•  Thyme 15%
•  Parsley 14%
•  Cayenne 13% 
 
•  Mushroom* 13%
•  Soy Sauce* 11% 
 
•  Cumin 11%
•  Mustard 10% 
 
•  Ginger 10% 
 
•  Bay Leaves 10% 
 
•  Oregano 10%
*indicates umami-rich ingredients
 
 
Beef and the Restaurant Scene
 
Today’s Menu Special: Beef Flavored with…
Onion
Cheese*
Mushroom*
Wine*
Garlic
Pepper/Peppercorns
Truffles*
Peppers/Green Peppers
Shallots
Bacon*
Barbecue Sauce*
Horseradish
Béarnaise
Mustard
*indicates umami-rich ingredients
 
 
http://www.beefandvealculinary.com/beefflavorp
airings.aspx
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Flavor plays a crucial role in meat quality perception, with tenderness and juiciness following closely behind. Consumers prioritize specifically cooked meat flavor for acceptance. Understanding flavor compounds and the difference between taste and flavor is vital in meat palatability. The five basic tastes - sweet, salty, sour, bitter, and umami - impact our survival, with umami recognized as the fifth taste due to its meaty and savory characteristics.

  • Flavor
  • Meat Science
  • Taste
  • Umami
  • Perception

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  1. Meat Flavor Jerrad Legako Graduate Student, Meat Science ANSC 3404

  2. IMPORTANCE OF FLAVOR Consumer perception of meat quality After appearance and tenderness flavor is most important Specifically cooked meat flavor Flavor is a key factor for acceptance Understanding flavor is therefore crucial Analysis of flavor compounds (Machiels and Istasse)

  3. MEAT PALATABILITY Tenderness Juiciness Flavor Flavor often considered the most important when tenderness is acceptable Result of volatile compounds produced during cooking

  4. TASTE vs. FLAVOR Taste refers to the five basic receptors: sweet, salty, sour, bitter and umami Flavor is the perception of chemical compounds reacting with receptors in the oral and nasal cavities (aroma) in combination with taste

  5. Perception of Flavor (Taste and Aroma) Aroma Detected in nose Small, volatile, fat soluble molecules Olfactory receptors Anterior nares Posterior nares Taste Detected on tongue and in mouth Large, water soluble molecules Tongue Taste receptors (L. J. Farmer)

  6. TASTE Salt and sour receptors are well understood while bitter and sweet receptors appear to be more complex.

  7. UMAMI Umami [oo-MOM-ee], known as the fifth taste, is described as meaty and savory or delicious derived from umai, the Japanese word for delicious Glutamates the salts of an amino acid - and other small molecules called nucleotides Although umami has been known for quite awhile, recently umami receptors have been clearly identified so this is a bona fide fifth taste.

  8. 5 TASTES Detection of these five tastes has been key to our survival throughout the ages Sweet means energy-giving carbohydrates Salt indicates essential minerals for life-sustaining cell functions and wound healing Sour says to proceed with caution, since many foods sour as they deteriorate. Umami signifies life-giving protein. And bitter warns spit it out, don t touch it because many natural toxins taste bitter

  9. FLAVOR CHEMISTRY What is flavor chemistry? A study of compounds which elicit a chemosensation or flavor sensation What produces flavor sensations? Chemical compounds reacting with receptors in the oral and nasal cavities Specifically ingested volatile compounds (Christen and Smith)

  10. FLAVOR CHEMISTRY Over 200 flavor compounds associated with cooked beef Sulferous and carbonyl compounds are predominate contributors Maillard reaction products End products result from sugars and amino groups Lipid breakdown products Higher concentrations may produce undesirable flavors (Calkins and Hodgen)

  11. MAIN FLAVOR FORMING REACTIONS Aroma compounds,e.g. Precursors N Reducing sugars, nucleotides, amino acids, peptides CH3S CHO CH3 Maillard reaction S NH O SH S S O O O SH S S Thermal degradation Thiamine O O O Thermal oxidation Lipids, fatty acids CHO O CHO O O HEAT (L.J. Farmer)

  12. MAILLARD REACTION Reducing sugar and free amino acid Results in the production of H2O, Therefore reaction does not occur with moist heat cookery reducing sugar + amine = brown pigments + flavors

  13. Flavor Compounds Formation by Maillard Reaction Reducing Sugars and -amino acids N-glycosylamine or N-fructosylamine 1-Amino-1-deoxy-2-ketose (Amadori intermediate) or 2-Amino-2-deoxy-1-aldose (Heynes intermediate) Reductones and Dehydroreductones H2S NH3 Amino Acids Strecker degradation + + Retroaldol Reaction Glyoxal Pyruvaldehyde Glycerolaldehyde Strecker Aldehydes + CO2+ -aminoketone (Methional, NH3, H2S) Furans Thiophenes Pyrroles Hydroxyacetone Hydroxyacetylaldehyde Acetoin Acetylaldehyde Heterocyclizaion Thiazoles Pyrroles Pyrazines Pyridines Oxazoles

  14. FLAVOR COMPOUND ANALYSIS Volatile compounds must be extracted Head space solid phase microextraction (HS-SPME) Separation of compounds may be done via gas chromatography (GC) Compounds may then be identified and sensory evaluated Identified: purified standards and/or mass spectral library Sensory evaluation: olfactometry (Frank and others)

  15. HS-SPME/GC-MS-Olfactory

  16. DISCUSSION Flavor is important to overall palatability of meat Flavor compounds may be studied by chemical and sensory evaluation Maillard reaction and lipid breakdown products are dependent on the type and condition of meat

  17. FACTORS AFFECTING MEAT FLAVOR Species Red meat species and poultry Breed Bos indicus vs Bos taurus? Sex Androstenone, skatole Diet Grain-fed vs. Grass-fed Age Young lamb vs. mutton Packaging MAP, over-wrap, vacuum

  18. FACTORS AFFECTING MEAT FLAVOR Fat Amount and type Muscle Location effect Aging Dry vs. wet Enhancement Brine solution containing salt Cooking method Dry vs. moist heat Degree of doneness

  19. Top 20 Beef Flavor Pairings in Newsstand Beef Recipes Onion 60% Garlic 55% Tomato* 32% Beef Broth/Stock/Bouillon* 25% Wine (mainly red)* 20% Cheese (mainly Parmesan, Cheddar, Blue)* 19% Sugar 18% Cream 18% Bell Pepper 16% Vinegar 16% Thyme 15% Parsley 14% Cayenne 13% Mushroom* 13% Soy Sauce* 11% Cumin 11% Mustard 10% Ginger 10% Bay Leaves 10% Oregano 10% *indicates umami-rich ingredients

  20. Beef and the Restaurant Scene Today s Menu Special: Beef Flavored with Onion Cheese* Mushroom* Wine* Garlic Pepper/Peppercorns Truffles* Peppers/Green Peppers Shallots Bacon* Barbecue Sauce* Horseradish B arnaise Mustard *indicates umami-rich ingredients

  21. http://www.beefandvealculinary.com/beefflavorp airings.aspx

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