The experiment aims to determine the concentration of an unknown copper (II) sulfate solution by measuring light absorption at different wavelengths. Colored solutions absorb specific light wavelengths and appear colored to our eyes. By creating a calibration curve and following Beer's Law, the conc

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Explore the various techniques involved in the characterization of DNA, including spectrophotometric assay, melting temperature determination, quantitative and qualitative analyses, DNA purity assessment, GC content calculation, and DNA denaturation processes. Learn how to determine DNA concentratio

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Protein estimation plays a vital role in various research applications. This practical guide covers the importance of determining protein concentration, specificity, sensitivity of methods, familiar spectrophotometric methods, standard curves, and determining unknown concentrations using Biuret meth

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This experiment aims to determine the concentrations of manganese and chromium in steel samples by converting Cr3+ and Mn2+ ions to light-absorbing forms, followed by spectrophotometric measurements at specific wavelengths. Steel samples are oxidized, dissolved, and further oxidized to form dichroma

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Quantitative determination of microbial growth is crucial for various purposes, with two commonly used methods being the standard plate count and spectrophotometric measurement. The standard plate count method estimates living microbial cell density, while spectrophotometric measurement relies on tu

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Learn about Agarose Gel Electrophoresis for DNA separation, Spectrophotometric method for determining DNA concentration, equipment required, buffer types, DNA markers for size identification, and applications in DNA analysis including paternity testing and crime scene investigation.

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