Protein desalting is crucial for purifying proteins after an ammonium sulfate precipitation step, which may leave the protein in a high-salt buffer. Three common desalting methods include dialysis using semi-permeable membranes, gel filtration with Sephadex G25 to exclude salts, and ultrafiltration

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Gel filtration chromatography is a molecular size-based separation technique used to separate macromolecules of different sizes. This method, also known as gel permeation or size exclusion chromatography, utilizes a porous stationary phase where small molecules penetrate all pores and are retained,

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