Understanding Recombinant DNA and Gene Cloning
Recombinant DNA is artificially created by combining DNA from multiple sources into a single molecule. This process involves treating DNA with restriction endonucleases, such as BamHI, which cut at specific sites, resulting in sticky ends that can base pair with complementary DNA molecules. DNA liga
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Understanding Enzymes and Restriction Enzymes in Molecular Biology
Enzymes are essential proteins that catalyze biochemical reactions. Bacteria have evolved enzymes like restriction endonucleases to protect against foreign DNA, such as viral DNA. The discovery of restriction enzymes in E. coli led to the Nobel Prize in Medicine in 1978. These enzymes cut DNA at spe
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Understanding Restriction Enzymes: The Key Players in DNA Manipulation
Restriction enzymes play a crucial role in cutting DNA molecules at specific points, allowing for precise genetic manipulation. This system involves host-controlled restriction and modification mechanisms to defend against foreign DNA, ensuring DNA integrity. Endonucleases recognize specific sequenc
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