Protein Sample Clean-Up Methods for MALDI Analysis
Protein sample clean-up for MALDI involves removing various contaminants like buffer, salts, urea, guanidine, EDTA, glycerol, DMSO, and detergents through methods such as dilution, washing, drop dialysis, cation exchange, and solid phase extraction using Zip tips. The process aims to reduce interfer
0 views • 8 slides
Understanding Differential Leucocyte Count for Blood Disorders
Differential Leucocyte Count (DLC) is crucial for diagnosing blood-related disorders by analyzing the distribution and morphology of white blood cells. Dr. Versha Prasad explains the significance, normal values, and various conditions like neutrophilia, lymphocytosis, monocytosis, and basophilia. Th
1 views • 15 slides
Overview of Veterinary Clinical Pathology and Importance of Anticoagulants
Veterinary clinical pathology involves the laboratory diagnostics and analysis of bodily fluids and tissues to support the diagnosis, treatment, and prevention of diseases. This field encompasses interpreting blood tests, blood smear examinations, and the use of various anticoagulants in specimen co
2 views • 46 slides
Understanding Anticoagulants in Laboratory Hematology
Anticoagulants are crucial additives that prevent blood clotting during laboratory procedures. The commonly used anticoagulants include EDTA, sodium citrate, and heparin, each with specific concentrations, modes of action, and color-coded identifications. EDTA is ideal for blood cell counts, sodium
0 views • 6 slides
Understanding Anticoagulants Used in Hematology Laboratory
Anticoagulants play a crucial role in hematology by preventing blood clotting, allowing separation of blood components, with EDTA being the most commonly used anticoagulant. Proper blood-to-anticoagulant ratio is essential to avoid inaccuracies in results, such as falsely reduced hematocrit or erron
0 views • 10 slides
Understanding Chelation Chemistry: Structural Requirements, Ligands, and Applications
Chelation chemistry involves the formation of specific complexes known as chelates, characterized by ligands that coordinate with a central metal ion. This article explores the structural requirements for chelate formation, the role of chelating agents like EDTA and DMG, and the difference between m
0 views • 27 slides