Investigating Dehydrogenase Activity Using Yeast
This investigation focuses on exploring respiration in yeast by tracking the breakdown of glucose using dehydrogenase enzymes. The experiment involves immobilizing yeast into gel balls and measuring the change in color of resazurin dye to quantify the rate of respiration. Safety measures, risk asses
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Understanding Dehydrogenase Enzymes and Their Activities
Dehydrogenases are intracellular respiratory enzymes that play a crucial role in oxidation-reduction reactions by transferring hydrogen atoms. This article explores the functions and properties of dehydrogenase enzymes, including the procedure to demonstrate their activity in a plant sample. Lactate
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Protein Isolation Techniques: Salting In and Salting Out
Proteins exhibit varying solubility based on salt concentrations, with salting in increasing solubility and salting out causing precipitation. This practical involves isolating lactate dehydrogenase enzyme using salting in, salting out, and dialysis techniques, as well as assessing purity through ac
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Newborn Screening 2014: Overview of Conditions and Management
The Pediatric Continuity Clinic Curriculum for Newborn Screening 2014 aims to increase familiarity with disorders tested in Florida Newborn Screening and manage abnormal results effectively. The presentation covers the goal of NBS, common diagnoses, resources for managing abnormal screens, and speci
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Understanding G6PD Deficiency Hemolytic Anemia: Biochemical Basis and Implications
G6PD deficiency hemolytic anemia is an inherited condition characterized by reduced levels of glucose-6-phosphate dehydrogenase (G6PD), leading to impaired production of NADPH. This deficiency affects the Pentose Phosphate Pathway (PPP) and can result in oxidative stress, causing damage to DNA, prot
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Biochemical Methodology 530 - Lactate Dehydrogenase Extraction and Purification Experiment
This course focuses on the extraction and purification of lactate dehydrogenase (LDH) using a series of procedures involving tissue preparation, centrifugation, and ammonium sulfate precipitation. The methodology includes detailed steps from preparing the extraction buffer to isolating LDH in differ
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