Enhancing Food Safety Standards and Crisis Preparedness

 
Project: 
T
echnical assistance to improve implementation of food safety
standards and disease crisis preparedness
 
Training course:
 
Sampling and labelling of samples,
sampling equipment and material, transport of samples
Lecturer:
 
Prof. Dr. Naim Deniz AYAZ
 
Date: … September 2022
Place: Nicosia,
 
Cyprus
 
Disclaimer: This presentation has been produced with the financial support of the European Union. Its contents are the sole
responsibility of NSF Euro Consultants Consortium – Contractor, and do not necessarily reflect the views of the European Union.
 
Project funded by the European Union Aid Programme for the Turkish Cypriot community,
implemented by 
NSF Euro Consultants Consortium
 
CONTENT
 
A: Sampling
B: Transport of samples
 
A: Sampling and labelling of samples
 
1A. 
Relevant Community rules
 about sampling
 
Regulation (EC) No 2073/2005
Chapter 3. Rules for sampling and preparation of test samples
3.1 General rules for sampling and preparation of test samples
In the absence of more specific rules on sampling and preparation of test
samples, the relevant standards of the ISO (International Organisation for
Standardisation) and the guidelines of the Codex Alimentarius shall be
used as reference methods.
 
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Codex Alimentarius: General guidelines on sampling, CAC/GL 50-2004
ISO/DIS 7218: Microbiology of food and animal feeding stuffs - General
rules for microbiological examinations.
ISO 17604: Microbiology of food and animal feeding stuffs - Carcass
sampling for microbiological analysis.
ISO 18593: Microbiology of food and animal feeding stuffs - Horizontal
methods for sampling techniques from surfaces using contact plates and
swabs.
NMKL (Nordic Committee on Food Analysis) Procedure No. 12: 
Guide on
Sampling for Analysis of Foods
. www.nmkl.org
 
2A. 
Sampling strategy
 
It 
is a 
planned procedure for selecting samples from a population and
 
for
conducting the sampling to obtain the information needed
.
 
A key issue is determining what strategy is used to select the units of the
target population to be subject to controls.
 
Objective sampling
 
Selective sampling
 
Suspect sampling
 
Objective sampling
 
A planned strategy based on the selection of a random sample, which is
statistically representative of the population to be analyzed.
 
This strategy provides data from which statistical inference can be
implemented. This could be applied to a convenient selection of food
retailers for hygiene controls
 
E
xample: 
Sampling of 
fresh poultry carcasse 
at retail level for detection
of 
Salmonella
 
to determine the prevalence at a specific point of the food
chain.
 
Selective sampling
 
A planned strategy where the selection of the sample is from previously
defined “high-risk” population groups.
 
The sampling procedure can be random or not.
 
If the sample is drawn randomly to be representative of the population
analysed, the results can be applied to the whole of this population.
 
E
xample: 
Sampling of high-risk products, for instance vacuum-packed
cold-smoked fish product supporting the growth of 
Listeria
monocytogenes 
during its shelf-life for detection and/or enumeration of
L. monocytogenes 
to determine the rate of contamination and/or the
level of contamination.
 
Suspect sampling
 
A selection of samples, where the units are selected based on the
judgement and experience regarding the population, lot, or sampling
frame.
 
The samples obtained from this procedure are not randomly extracted.
 
E
xample: 
Sampling carried out as a part of a food-borne outbreak
investigation or where an inspection indicates there may be a food safety
problem or where a HACCP plan review results in concerns regarding
potential food safety problems.
 
3
A
.
 
A
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m
 
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m
p
l
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n
g
 
D
ifferent sampling regimes may be required
 for each aim
 
to verify the compliance with Regulation (EC) No 2073/2005,
to verify the microbiological safety of food 
(
no microbiological criteria
)
to obtain general information on the microbiological status
to observe carefully one or more food businesses verifying their food
safety management systems
t
o check the compliance of individual batches
to investigate suspected food-borne outbreaks, complaints etc.
to identify and obtain information on new or emerging microbiological
hazards, generating data for risk profiles and risk assessments.
 
4A. 
Sampling plan
 
T
wo-class sampling plan
Satisfactory
U
nsatisfactory,
based on one limit value ‘m=M
 
T
hree-class sampling plan
S
atisfactory
A
cceptable
U
nsatisfactory.
 
A three-class sampling plan is used
if it is acceptable that some
samples exceed the lower limit (m),
as long as a risk contamination level
(M) is not exceeded
.
 
In official sampling the sampling plans described in 
Regulation (EC) No 2073/2005
should be used, as a minimum, when the acceptability of a food batch or a process is
assessed.
 
For any sampling plan there is probability of accepting a batch which may be unacceptable
 
E
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p
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n
:
 
5 sample units (n=5) are tested and
no 
Salmonella
 positive sample unit is permitted (c=0)
 
There is a:
90 % chance of accepting a batch containing 2 % 
Salmonella
 positive units.
77 % chance of accepting a batch containing 5 % 
Salmonella
 positive units.
59 % chance of accepting a batch containing 10 % 
Salmonella
 positive units.
17 % chance of accepting a batch containing 30 % 
Salmonella
 positive units.
3 % chance of accepting a batch containing 50 % 
Salmonella
 positive units.
 
5A.
 Sampling frequency
 
R
egulation (EC) No 2073/2005 
sets down a fixed weekly sampling
frequency for the 
FBOs 
for certain products:
carcases
minced meat
meat preparations
mechanically separated meat
 
In other cases the 
FBOs 
have to decide the sampling frequency on a case-
by-case basis taking into account the risk related to their products.
 
O
fficial controls no fixed sampling and testing frequency
C
ompetent authorities are planning their sampling  strategy and aim of
sampling according to 
Article 41 of Regulation (EC) No 882/2004.
 
B: Transport of Samples
 
Standardized procedures for the transport of samples to the laboratory,
the storage and the starting of the analysis are presented in
ISO/DIS 7218: Microbiology of food and animal feeding stuffs - General
rules for microbiological examinations.
 
ISO/DIS 7218: Microbiology of food and animal feeding stuffs -
General rules for microbiological examinations
 
does not set a maximum limit for the time of transportation of products not
stable at 
ambient temperature
.
However, given the potential for change in the levels of the target organisms
 -
sample should 
arrive at the laboratory within 36 hours after sampling
.
M
icrobiological analysis should be started 
as soon as possible after receipt at
laboratory, preferably 
within 24 hours
.
It is recommended that analysis is started, as a rule, 
within 48 hours of taking
the sample
, unless the testing protocol specifically states otherwise.
 
Transportation of R
efrigerated 
P
roducts
 
For highly perishable fresh, refrigerated
products:
During transport and storage 
freezing
temperatures must be avoided
.
Pre-packed food should be stored 
at or
below the storage temperature 
given
on the label. given on the label.
 
In case of examination at a later stage, e.g. for checking compliance of a
prepacked food at the use by date the samples should be stored at the
laboratory under the recommended conditions given on the label.
 
CONTACT
 
THANK YOU FOR YOUR ATTENTION
 
P
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Project funded by the European Union Aid Programme for the Turkish Cypriot community,
implemented by 
NSF Euro Consultants Consortium
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This project focuses on improving the implementation of food safety standards and enhancing disease crisis preparedness through a training course on sampling, labeling, and transportation of samples. The course, conducted by Prof. Dr. Naim Deniz AYAZ, covers relevant regulations and internationally recognized standards for sampling, emphasizing the importance of proper sampling strategies. Funded by the European Union, this initiative aims to strengthen food safety practices in Nicosia, Cyprus.


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  1. Project: Technical assistance to improve implementation of food safety standards and disease crisis preparedness Training course: Sampling and labelling of samples, sampling equipment and material, transport of samples Lecturer: Prof. Dr. Naim Deniz AYAZ Date: September 2022 Place: Nicosia, Cyprus Project funded by the European Union Aid Programme for the Turkish Cypriot community, implemented by NSF Euro Consultants Consortium Disclaimer: This presentation has been produced with the financial support of the European Union. Its contents are the sole responsibility of NSF Euro Consultants Consortium Contractor, and do not necessarily reflect the views of the European Union.

  2. CONTENT A: Sampling B: Transport of samples

  3. A: Sampling and labelling of samples 1A. Relevant Community rules about sampling

  4. Regulation (EC) No 2073/2005 Chapter 3. Rules for sampling and preparation of test samples 3.1 General rules for sampling and preparation of test samples In the absence of more specific rules on sampling and preparation of test samples, the relevant standards of the ISO (International Organisation for Standardisation) and the guidelines of the Codex Alimentarius shall be used as reference methods.

  5. Internationally Internationally recognised recognised standards standards and and guide guide for sampling for sampling Codex Alimentarius: General guidelines on sampling, CAC/GL 50-2004 ISO/DIS 7218: Microbiology of food and animal feeding stuffs - General rules for microbiological examinations. ISO 17604: Microbiology of food and animal feeding stuffs - Carcass sampling for microbiological analysis. ISO 18593: Microbiology of food and animal feeding stuffs - Horizontal methods for sampling techniques from surfaces using contact plates and swabs. NMKL (Nordic Committee on Food Analysis) Procedure No. 12: Guide on Sampling for Analysis of Foods. www.nmkl.org

  6. 2A. Sampling strategy It is a planned procedure for selecting samples from a population and for conducting the sampling to obtain the information needed. A key issue is determining what strategy is used to select the units of the target population to be subject to controls. Objective sampling Selective sampling Suspect sampling

  7. Objective sampling A planned strategy based on the selection of a random sample, which is statistically representative of the population to be analyzed. This strategy provides data from which statistical inference can be implemented. This could be applied to a convenient selection of food retailers for hygiene controls Example: Sampling of fresh poultry carcasse at retail level for detection of Salmonella to determine the prevalence at a specific point of the food chain.

  8. Selective sampling A planned strategy where the selection of the sample is from previously defined high-risk population groups. The sampling procedure can be random or not. If the sample is drawn randomly to be representative of the population analysed, the results can be applied to the whole of this population. Example: Sampling of high-risk products, for instance vacuum-packed cold-smoked fish product supporting monocytogenes during its shelf-life for detection and/or enumeration of L. monocytogenes to determine the rate of contamination and/or the level of contamination. the growth of Listeria

  9. Suspect sampling A selection of samples, where the units are selected based on the judgement and experience regarding the population, lot, or sampling frame. The samples obtained from this procedure are not randomly extracted. Example: Sampling carried out as a part of a food-borne outbreak investigation or where an inspection indicates there may be a food safety problem or where a HACCP plan review results in concerns regarding potential food safety problems.

  10. 3A. 3A. Aim of sampling Aim of sampling Different sampling regimes may be required for each aim to verify the compliance with Regulation (EC) No 2073/2005, to verify the microbiological safety of food (no microbiological criteria) to obtain general information on the microbiological status to observe carefully one or more food businesses verifying their food safety management systems to check the compliance of individual batches to investigate suspected food-borne outbreaks, complaints etc. to identify and obtain information on new or emerging microbiological hazards, generating data for risk profiles and risk assessments.

  11. 4A. Sampling plan For any sampling plan there is probability of accepting a batch which may be unacceptable Two-class sampling plan Satisfactory Unsatisfactory, Three-class sampling plan Satisfactory Acceptable Unsatisfactory. based on one limit value m=M A three-class sampling plan is used if it is acceptable that some samples exceed the lower limit (m), as long as a risk contamination level (M) is not exceeded. In official sampling the sampling plans described in Regulation (EC) No 2073/2005 should be used, as a minimum, when the acceptability of a food batch or a process is assessed.

  12. Example Example for for two two class sampling plan class sampling plan: : 5 sample units (n=5) are tested and no Salmonella positive sample unit is permitted (c=0) There is a: 90 % chance of accepting a batch containing 2 % Salmonella positive units. 77 % chance of accepting a batch containing 5 % Salmonella positive units. 59 % chance of accepting a batch containing 10 % Salmonella positive units. 17 % chance of accepting a batch containing 30 % Salmonella positive units. 3 % chance of accepting a batch containing 50 % Salmonella positive units.

  13. 5A. Sampling frequency Regulation (EC) No 2073/2005 sets down a fixed weekly sampling frequency for the FBOs for certain products: carcases minced meat meat preparations mechanically separated meat In other cases the FBOs have to decide the sampling frequency on a case- by-case basis taking into account the risk related to their products. Official controls no fixed sampling and testing frequency Competent authorities are planning their sampling strategy and aim of sampling according to Article 41 of Regulation (EC) No 882/2004.

  14. B: Transport of Samples Standardized procedures for the transport of samples to the laboratory, the storage and the starting of the analysis are presented in ISO/DIS 7218: Microbiology of food and animal feeding stuffs - General rules for microbiological examinations.

  15. ISO/DIS 7218: Microbiology of food and animal feeding stuffs - General rules for microbiological examinations does not set a maximum limit for the time of transportation of products not stable at ambient temperature. However, given the potential for change in the levels of the target organisms - sample should arrive at the laboratory within 36 hours after sampling. Microbiological analysis should be started as soon as possible after receipt at laboratory, preferably within 24 hours. It is recommended that analysis is started, as a rule, within 48 hours of taking the sample, unless the testing protocol specifically states otherwise.

  16. Transportation of Refrigerated Products For highly perishable fresh, refrigerated products: During transport and storage freezing temperatures must be avoided. Pre-packed food should be stored at or below the storage temperature given on the label. given on the label. In case of examination at a later stage, e.g. for checking compliance of a prepacked food at the use by date the samples should be stored at the laboratory under the recommended conditions given on the label.

  17. CONTACT Project e-mail: foodsafetyprojectTCc@gmail.com THANK YOU FOR YOUR ATTENTION Project funded by the European Union Aid Programme for the Turkish Cypriot community, implemented by NSF Euro Consultants Consortium

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